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To get a quote for your project as quickly as possible, please gather the following information and send your inquiries to:

For tGBS® Genotyping-by-Sequencing Projects

Number of samples you would like to submit as a single "batch"

Name of species you are working on and whether there is an assembled reference genome available for SNP calling

If there is no sequenced reference genome, do you know the approximate genome size of the target species? If not, don't worry, our sales associate will be happy to work with you to track down or generate an estimated size1

Are your samples expected to be fully inbred (ie recombinant inbred lines, doubled haploids, etc.) or do you expect heterozygosity in some or all of your samples?2

Approximate goal of the project. You can say things like: "Map QTLs", "Conduct GWAS", "Study population structure or gene flow"3

Write to to inquire about our tGBS® genotyping services

1 The reason we ask about genome size is that our goal with tGBS® genotyping is to target a certain median number of reads per sample per sequenced site in the genome. At the same level of genome bracket reduction, larger genomes require more total sequencing per sample to hit that target coverage, which influences how we will design your experiment and the ultimate cost of the project.

2 In principle, segregating markers in fully homozygous lines can be genotyped with as little as one aligned read per site per sample. We still require more reads than that to ensure we're providing the most accurate genotype calls possible. However, genotyping non-homozygous samples with parameters tuned for homozygous samples can produce error rates >6% in loci with 5 or fewer aligned reads per sample.

3 The reason we ask about the type of project you are doing is that it influences the number of high quality segregating markers needed. For a QTL study, we can target a much smaller subset of the genome, bracket reducing sequencing costs while keeping the median number of reads per site per sample high, which allows us to charge lower per sample prices. For a GWAS study where 10,000s or 100,000s of markers might be needed, it is necessary to target more sites in the genome and generate more sequence data per sample.

For Other Custom Projects and Breeding Programs

If you are in need of services related to genotyping, mapping, or breeding that are not described in our services page, please don't hesitate to contact us at with a brief description of your project. Our friendly staff will be more than happy to work with you and propose a project plan suitable for your experiment.