tGBS® › Tunable Genotyping-by-sequencing mapping service

Our proprietary tGBS® technology is an alternative to conventional genotyping by sequencing that offers several significant advantages. Because our technology allows us to more stringently target reads to a smaller number of sites in the genome, SNP calls are more accurate and have less missing data, while heterozygous loci can be genotyped directly without a need for imputation. The greater proportion of sites which are directly genotyped and the greater read depth at these sites increases the power of tGBS to discover rare alleles. In addition tGBS can be conducted with or without a reference genome.

tGBS can be used to facilitate marker discovery, for mapping genes or QTL in F2, F1BC1, or other inbred or non-inbred populations, for conducting genome wide association studies, and to enable genomic selection (GS) based breeding programs.

For most projects we deliver our report and finalized SNP calls within eight weeks of receiving your samples at our Iowa facility, however rush projects can be completed in at little as 4-6 weeks.

You can read about how to submit either DNA or tissue samples for tGBS genotyping here.

Please write to us at with any questions about the services we can provide or to request a quote.

Optional Features:

  • Our project reports are both comprehensive and user-friendly but some customers appreciate the opportunity for additional one-on-one consultation with one of our experts.

Resources:

tGBS Slides: Data2Bio-tGBS.pdf

Videos & Workshops:

Overview of tGBS - Genotyping by Sequencing on Ion Proton™ System In-depth description of tGBS - tGBS®: A Next Generation Genotyping-by-Sequencing Technology Fast Turn-Around genotyping without a Reference Genome

Client Evaluation of tGBS: The Fang lab employed both conventional GBS and tGBS for genotyping in tetraploid cotton and validated markers discovered by each system. Click here to download their paper published in The Plant Genome to see how the accuracy and utility of the two techniques compared.
Accurate Heterozygous SNP Calling in Action: A manuscript published in Genome Biology describes how tGBS genotyping of an F1 mapping population of yellow catfish (Tachysurus fulvidraco) was used to construct separate maternal and paternal genetic maps which were used to order genomic scaffolds into pseudomolecules. [ Tang et. al., 2015, Genome Biology ].