tGBS SNP Genotyping

tGBS® SNP Genotyping


tGBS® is an efficient and fast genotyping platform for mapping populations and diversity panels


Our proprietary tGBS® technology is an alternative to conventional genotyping by sequencing that offers several significant advantages. Because our technology allows us to more stringently target reads to a smaller number of sites in the genome, SNP calls are more accurate and have less missing data, while heterozygous loci can be genotyped directly without a need for imputation. The greater proportion of sites which are directly genotyped and the greater read depth at these sites increases the power of tGBS® to discover rare alleles. In addition tGBS® can be conducted with or without a reference genome.

tGBS® can be used to facilitate marker discovery, for mapping genes or QTL in F2, F1BC1, or other inbred or non-inbred populations, for conducting genome wide association studies, and to enable genomic selection (GS) based breeding programs.

For most projects we deliver our report and finalized SNP calls within eight weeks of receiving your samples at our Iowa facility, however rush projects can be completed in at little as 4-6 weeks.

You can read about how to submit either DNA or tissue samples for tGBS® genotyping here.

Optional Features: Our project reports are both comprehensive and user-friendly but some customers appreciate the opportunity for additional one-on-one consultation with one of our experts.


Reports

Our project reports are both comprehensive and user-friendly but some customers appreciate the opportunity for additional one-on-one consultation with one of our experts.


Presentation

Click here to download slides briefly describing Data2Bio's tGBS® technology.


Videos & Workshops

PAG2015 - tGBS: Fast-Turn-Around

Fast Turn-Around genotyping without a Reference Genome

Play Video

Download Video


PAG2014 - tGBS: In-Depth Description

In-depth description of tGBS®: A Next Generation Genotyping-by-Sequencing Technology

Play Video

Download Video


tGBS-enabled Genomic Selection

Overview of tGBS®: Genotyping by Sequencing on Ion Proton System

Play Video

Download Video


Learn more about tGBS®: Read Ott et al's description of the tGBS® method, with example datasets from mini-diversity panels, RILs, and F2 lines and head to head comparisons with conventional genotyping by sequencing (cGBS) at bioRxiv


Accurate Heterozygous SNP Calling in Action: A manuscript published in Genome Biology describes how tGBS® genotyping of an F1 mapping population of yellow catfish (Tachysurus fulvidraco) was used to construct separate maternal and paternal genetic maps which were used to order genomic scaffolds into pseudomolecules. [ Tang et. al., 2015, Genome Biology ]


Questions? For questions regarding our services and/or other matters, please contact us at: