To get a quote for your project as quickly as possible, please gather the following information and send your inquiries to:
Number of samples you would like to submit as a single "batch"
Name of species you are working on and whether there is an assembled reference genome available for SNP calling
If there is no sequenced reference genome, do you know the approximate genome size of the target species? If not, don't worry, our sales associate will be happy to work with you to track down or generate an estimated size1
Are your samples expected to be fully inbred (ie recombinant inbred lines, doubled haploids, etc.) or do you expect heterozygosity in some or all of your samples?2
Approximate goal of the project. You can say things like: "Map QTLs", "Conduct GWAS", "Study population structure or gene flow"3
Write to to inquire about our tGBS® services
1 The reason we ask about genome size is that our goal with tGBS® is to target a certain median number of reads per sample per sequenced site in the genome. At the same level of genome bracket reduction, larger genomes require more total sequencing per sample to hit that target coverage, which influences how we will design your experiment and the ultimate cost of the project.
2 In principle, segregating markers in fully homozygous lines can be genotyped with as little as one aligned read per site per sample. We still require more reads than that to ensure we're providing the most accurate genotype calls possible. However, genotyping non-homozygous samples with parameters tuned for homozygous samples can produce error rates >6% in loci with 5 or fewer aligned reads per sample.
3 The reason we ask about the type of project you are doing is that it influences the number of high quality segregating markers needed. For a QTL study, we can target a much smaller subset of the genome, bracket reducing sequencing costs while keeping the median number of reads per site per sample high, which allows us to charge lower per sample prices. For a GWAS study where 10,000s or 100,000s of markers might be needed, it is necessary to target more sites in the genome and generate more sequence data per sample.
What is the genetic background of your samples (DNA or RNA) and traits (qualitative or quantitative)?4
Name of the species and whether or not there is an assembled reference genome you would like to be used for gene mapping
If there is no sequenced reference genome, we would need to perform a custom transcriptome assembly
Population structure and details
Is your population a F2, RIL, NIL, backcross, or other?
Will you have enough number of individuals (≥30) for each of the two poolings?5
Would you like to obtain more accurate transcriptome information (for RNA samples only)?6
Write to to inquire about our BSR-Seq and/or gBSA services
4 The reason we ask about genetic basis of your material is that the approaches between gBSA and BSR-Seq is slightly different and they were designed for qualitative traits. But they were also successfully used for quantitative trait if well designed. If you are not sure, we will be happy to provide consultation with your segregation information.
5 Population information will be used to design pooling strategy. Based on our experiences, minimum of 30 individuals in a pool is requibracket red to give more precise mapping intervals. However, less individuals may be acceptable if sample resource is limited. Please describe your concern that we can provide with some suggestion.
6 One of advantages of BSR-Seq is that it provides additional transcriptome information; however, while only one pooled library per phenotype is sufficient for mapping, replicated libraries are generally required to provide statistical power for confidently identifying differentially expressed genes. Our sale representative can give you an additional quote if requested.
If you are in need of services related to genotyping, mapping, or breeding that are not described in our services page, please don't hesitate to contact us at with a brief description of your project. Our friendly staff will be more than happy to work with you and propose a project plan suitable for your experiment.